Chenshuo Ma,1 Ludmila A. Kasatkina,2 Mikhail E. Matlashov,2 Tri Vu,1 Mucong Li,1 Andrii A. Kaberniuk,2 Junjie Yaohttps://orcid.org/0000-0002-2381-706X,1 Vladislav V. Verkhusha2
1Duke Univ. (United States) 2Albert Einstein College of Medicine (United States)
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Our study exploits Rhodopseudomonas palustris BphP1 bacterial phytochrome to generate a near-infrared (NIR) loxP-BphP1 photoswitchable transgenic mouse model that enables deep-tissue optogenetics and photoacoustic tomography (PAT). BphP1 incorporates biliverdin and reversibly switches between the ground state and activated state, with distinct optical absorption spectra in the NIR window. We validated the optogenetic performance of the BphP1-encoded mouse model to trigger gene transcription, and demonstrated its superior capability of deep-tissue optogenetics. Then, taking advantage of BphP1's photoswitching properties, we can suppress the non-switching signals from background blood and improve the molecular detection sensitivity of PAT by three orders of magnitude.
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Chenshuo Ma, Ludmila A. Kasatkina, Mikhail E. Matlashov, Tri Vu, Mucong Li, Andrii A. Kaberniuk, Junjie Yao, Vladislav V. Verkhusha, "Deep-tissue photoacoustic imaging and optogenetic manipulation of a near-infrared photoswitchable transgenic mouse model," Proc. SPIE PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023, PC123791A (9 March 2023); https://doi.org/10.1117/12.2648543