Longitudinal imaging of live organisms is essential to life science research aiming to understand biological processes. Recently, the imaging system has been housed for longitudinal imaging by mounting the optical imaging system in an incubator, while facing the limitations of single-modality and low throughput. Here, we proposed the OCToScope, a multi-modal, high-throughput and compact platform that is compatible with commercial incubators. OCToScope incorporates Optical Coherence Tomography(OCT) to achieve non-invasive three-dimensional imaging, and fluorescent imaging that retrieves cell-specific information. OCToScope also supports automatic scanning of mass samples quantity with motorized x-y-z stage and perform high time-resolution imaging for long-term studies. we used OCToScope to provide a systematic and comprehensive characterization of human heart organoids for over 30 days without any interference.
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