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Imaging of the interior of object with light has long been a challenge for optical imaging. Optical diffraction tomography (ODT) is able to obtain three-dimensional (3D) object information through object rotation. We will discuss harmonic optical tomography (HOT) that exploits a defocused illumination beam for nonlinear optical tomography. We will also discuss our demonstration of coherent ODT with incoherent light emission in a new optical tomography technique called fluorescent diffraction tomography (FDT) and the use of spatial frequency imaging for high speed nonlinear optical microscopy.
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Randy A. Bartels, Gabe Murray, Patrick Stockton, Ali Pezeshki, Jeff Field, Jeff Squier, "High speed spatial frequency modulation nonlinear spectroscopic microscopy and tomography," Proc. SPIE PC11971, High-Speed Biomedical Imaging and Spectroscopy VII, PC1197101 (2 March 2022); https://doi.org/10.1117/12.2613518