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7 March 2022 Imaging flow cytometer based on linear array spot illumination generated by diffractive optical elements
Yong Han, Jingjing Zhao, Zixi Chao, Zeheng Jiao, Attila Tárnok, Zheng You
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Proceedings Volume PC11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX; PC1196409 (2022) https://doi.org/10.1117/12.2607840
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
In this work, we developed a new method for high throughput imaging flow cytometry, using diffractive optics elements to generate linear laser spot array for illumination, and single-pixel detectors for detection. The illumination spots are arranged in a line at equal intervals and form a small angle with the direction of the cell movement. When the cell passes through the illumination area, the two-dimensional information of the cell's fluorescence and scattered intensity profile is encoded into signals detected by the PMTs. Fluorescence and scattering imaging were experimentally demonstrated for beads and cells traveling at a velocity of 4.7 m/s in a microfluidic chip, with a resolution of 1 μm and a maximum throughput of 5000cell/s.
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Yong Han, Jingjing Zhao, Zixi Chao, Zeheng Jiao, Attila Tárnok, and Zheng You "Imaging flow cytometer based on linear array spot illumination generated by diffractive optical elements", Proc. SPIE PC11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX, PC1196409 (7 March 2022); https://doi.org/10.1117/12.2607840
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KEYWORDS
Optical components
Luminescence
Signal detection
Flow cytometry
Image segmentation
Scattering
Image resolution
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