Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells

Björn Kemper; Daniel Carl; Alexander Höink; Gert von Bally; Ilona Bredebusch; Jürgen Schnekenburger

doi:10.1117/12.662781

14 April 2006 Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells

Björn Kemper, Daniel Carl, Alexander Höink, Gert von Bally, Ilona Bredebusch, Jürgen Schnekenburger

Author Affiliations +

Proceedings Volume 6191, Biophotonics and New Therapy Frontiers; 61910T (2006) https://doi.org/10.1117/12.662781
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

A modular digital holographic microscopy system for integration in commercial microscopes has been developed. The reconstruction of the digitally captured holograms is performed by application of a spatial phase shifting non diffractive reconstruction method. As a consequence of the applied algorithms the reconstructed holographic images contain not the disturbing terms "twin image" and "zero order". In combination with microscope lenses the system's lateral resolution is improved up to the diffraction limit. Digital holographic focus adjustment with constant imaging scale allows multi focus imaging of object planes and subsequent focus correction from only a single captured hologram. Results of investigations on technical specimen characterize the lateral and axial resolution of the system. The applicability of the system is demonstrated by results obtained from drug stimulated cellular morphology changes and apoptosis monitoring of living pancreas cells.

Citation Download Citation

Björn Kemper, Daniel Carl, Alexander Höink, Gert von Bally, Ilona Bredebusch, and Jürgen Schnekenburger "Modular digital holographic microscopy system for marker free quantitative phase contrast imaging of living cells", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910T (14 April 2006); https://doi.org/10.1117/12.662781

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