Paper
10 July 2003 Characterization of the second harmonic signal from collagen
Author Affiliations +
Abstract
Collagen is known to be a very effective generator of the second harmonic of incident light from 700 to 1100nm, and second harmonic generation (SHG) microscopy is coming into use as a tool for studying the distribution of collagen in tissue. It also shows promise as a technique for characterizing collagen - both in distinguishing different collagen types and their packing and in identifying degradation of collagen in pathologic conditions. However many aspects of image formation in SHG microscopy of collagen remain imperfectly understood, and we have commenced a rigorous study of these factors. The present paper presents the first results from this program.
© (2003) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Guy C. Cox, Paul Xu, Colin J. R. Sheppard, and John A. Ramshaw "Characterization of the second harmonic signal from collagen", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.485598
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Cited by 27 scholarly publications.
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KEYWORDS
Collagen

Lithium

Stereolithography

Microscopy

RGB color model

Second-harmonic generation

Biomedical optics

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