Application of diffusing wave spectroscopy for in-vitro blood testing and skin microcirculation measurements

Alexander N. Korolevich; Natali S. Dubina; Sergei I. Vecherinski; Igor Victorovich Meglinski

doi:10.1117/12.438425

22 August 2001 Application of diffusing wave spectroscopy for in-vitro blood testing and skin microcirculation measurements

Alexander N. Korolevich, Natali S. Dubina, Sergei I. Vecherinski, Igor Victorovich Meglinski

Author Affiliations +

Proceedings Volume 4427, Imaging of Tissue Structure and Function; (2001) https://doi.org/10.1117/12.438425
Event: Imaging of Tissue Structure and Function, 2001, none, Russian Federation

Abstract

The extension of the photon correlation spectroscopy in multiple scattering regimes, so-called diffusing wave spectroscopy (DWS) employed to the study of blood samples. Multiple scattered light from a Helium-Neon (He-Ne) laser beam incident on the blood samples was detected by a photomultiplier, and both the temporal autocorrelation intensity functions g₂((tau) ) and power spectra S((omega) ) were measured spectrumaanalyzer. The potential of using diffusing wave spectroscopy (DWS) for the qualitative and quantitative determining of the structural characteristics of the blood elements was studied experimentally. The experimental studies made permit stating to use DWS for the blood cells monitoring in a multiple scattering regime. This paper describes our initial attempts at applying DWS to the study of the blood samples of both healthy donors and patients with cardiac ischemia in vivo and in vitro. The subsequent experiments provide a verification of DWS of blood cells shape monitoring under multiple scattering. The second kind of the experimental application of DWS was the skin microcirculation study in vivo. The results of the mean resting capillary red blood cells (RBC) velocity for two patients with cardiovascular diseases are presented. Two maximums in the mean RBC velocity in time dependence of the He-Ne laser exposure are observed in all measurements.

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Alexander N. Korolevich, Natali S. Dubina, Sergei I. Vecherinski, and Igor Victorovich Meglinski "Application of diffusing wave spectroscopy for in-vitro blood testing and skin microcirculation measurements", Proc. SPIE 4427, Imaging of Tissue Structure and Function, (22 August 2001); https://doi.org/10.1117/12.438425

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