Rapid analysis of gene copy numbers in cancer by FISH and automated spot counting from stacked confocal images

Artyom M. Grigoryan; Edward R. Dougherty; Lukas Bubendorf; Juha Kononen; Ollie Kallionienmi

doi:10.1117/12.384245

26 April 2000 Rapid analysis of gene copy numbers in cancer by FISH and automated spot counting from stacked confocal images

Artyom M. Grigoryan, Edward R. Dougherty, Lukas Bubendorf, Juha Kononen, Ollie Kallionienmi

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Proceedings Volume 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation; (2000) https://doi.org/10.1117/12.384245
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Fluorescent in situ hybridization (FISH) is an excellent method for detection of gene copy number alterations in cancer and other diseases. A limitation of the technology is the tedious, inaccurate and often highly subjective spot counting. For a number of reasons, automation of FISH spot, counting has not been accomplished. FISH signals are often at different focal planes, resulting in interfering out-of- focus light. This paper describes current progress towards automated FISH spot counting, with particular reference to the previous technical limitations.

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Artyom M. Grigoryan, Edward R. Dougherty, Lukas Bubendorf, Juha Kononen, and Ollie Kallionienmi "Rapid analysis of gene copy numbers in cancer by FISH and automated spot counting from stacked confocal images", Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); https://doi.org/10.1117/12.384245

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KEYWORDS

Binary data

Image segmentation

Evolutionary algorithms

Image processing algorithms and systems

Cancer

Confocal microscopy

Tissues

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