Cell nuclei segmentation in noisy images using morphological watersheds

Jose Alfredo Ferreira Costa; Nelson D. A. Mascarenhas; Marcio Luiz de Andrade Netto

doi:10.1117/12.292759

30 October 1997 Cell nuclei segmentation in noisy images using morphological watersheds

Jose Alfredo Ferreira Costa, Nelson D. A. Mascarenhas, Marcio Luiz de Andrade Netto

Proceedings Volume 3164, Applications of Digital Image Processing XX; (1997) https://doi.org/10.1117/12.292759
Event: Optical Science, Engineering and Instrumentation '97, 1997, San Diego, CA, United States

Abstract

A major problem in image processing and analysis is the segmentation of its components. Many computer vision tasks process image regions after segmentation, and the minimization of errors is then crucial for a good automatic inspection system. This paper presents an applied work on automatic segmentation of cell nuclei in digital noisy images. One of the major problems when using morphological watersheds is oversegmentation. By using an efficient homotopy image modification module, we prevent oversegmentation. This module utilizes diverse operations, such as sequential filters, distance transforms, opening by reconstruction, top hat, etc., some in parallel, some in cascade form, leading to a new set of internal and external cell nuclei markers. Very good results have been obtained and the proposed technique should facilitate better analysis of visual perception of cell nuclei for human and computer vision. All steps are presented, as well as the associated images. Implementations wee done in the Khoros system using the MMach toolbox.

Citation Download Citation

Jose Alfredo Ferreira Costa, Nelson D. A. Mascarenhas, and Marcio Luiz de Andrade Netto "Cell nuclei segmentation in noisy images using morphological watersheds", Proc. SPIE 3164, Applications of Digital Image Processing XX, (30 October 1997); https://doi.org/10.1117/12.292759

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