Video analysis of polymerized micro-cantilever deflected by the laser trap

Zoltan Tomori; Veronika Kazikova; Jana Kubackova; Gregor Bánó

doi:10.1117/12.2529129

9 September 2019 Video analysis of polymerized micro-cantilever deflected by the laser trap

Zoltan Tomori, Veronika Kazikova, Jana Kubackova, Gregor Bánó

Proceedings Volume 11083, Optical Trapping and Optical Micromanipulation XVI; 1108336 (2019) https://doi.org/10.1117/12.2529129
Event: SPIE Nanoscience + Engineering, 2019, San Diego, California, United States

Abstract

Elastic micro-cantilever of 30-micrometer size is repeatedly deflected/released by optical tweezers trap, recorded by a high-speed camera (500 frames/sec) and subsequently processed off-line. This paper evaluates the position detection methods of the cantilever head, which is distorted by a diffraction pattern. We developed and tested four methods in our VideoAnalyser software - radial extremes, Hough transform, local corner tracking, and voting normal lines. The time dependence of the head position contains the information about the properties of both: cantilever material and the surrounding environment. Averaging of aligned graphs corresponding to individual cycles significantly improves the signal-to-noise ratio.

Citation Download Citation

Zoltan Tomori, Veronika Kazikova, Jana Kubackova, and Gregor Bánó "Video analysis of polymerized micro-cantilever deflected by the laser trap", Proc. SPIE 11083, Optical Trapping and Optical Micromanipulation XVI, 1108336 (9 September 2019); https://doi.org/10.1117/12.2529129

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