Special Section on Color in Texture and Material Recognition

Color features as an approach for the automated screening of Salmonella strain

[+] Author Affiliations
Alejandra Serrano Trujillo, Luis E. Palafox

Universidad Autónoma de Baja California, Facultad de Ciencias Químicas e Ingeniería, Calzada Universidad 14418, Tijuana 22390, México

Viridiana Contreras González

Centro de Investigación y Desarrollo de Tecnología Digital, Ave. Instituto Politécnico Nacional No. 1310, Tijuana 22435, México

Saulo E. Andrade Rincón

Laboratorio Microbiológico ANDA, Boulevard Gustavo Díaz Ordaz, Tijuana 22106, México

J. Electron. Imaging. 25(6), 061411 (Jul 21, 2016). doi:10.1117/1.JEI.25.6.061411
History: Received March 27, 2016; Accepted July 5, 2016
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Abstract.  We present the implementation of a feature extraction approach for the automated screening of Salmonella sp., a task visually carried out by a microbiologist, where the resulting color characteristics of the culture media plate indicate the presence of this strain. The screening of Salmonella sp. is based on the inoculation and incubation of a sample on an agar plate, allowing the isolation of this strain, if present. This process uses three media: Xylose lysine deoxycholate, Salmonella Shigella, and Brilliant Green agar plates, which exhibit specific color characteristics over the colonies and over the surrounding medium for a presumed positive interpretation. Under a controlled illumination environment, images of plates are captured and the characteristics found over each agar are processed separately. Each agar is analyzed using statistical descriptors for texture, to determine the presence of colonies, followed by the extraction of color features. A comparison among the color features seen over the three media, according to the FDA Bacteriological Analytical Manual, determines the presence of Salmonella sp. on a given sample. The implemented process proves that the task addressed can be accomplished under an image processing approach, leading to the future validation and automation of additional screening processes.

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Citation

Alejandra Serrano Trujillo ; Viridiana Contreras González ; Saulo E. Andrade Rincón and Luis E. Palafox
"Color features as an approach for the automated screening of Salmonella strain", J. Electron. Imaging. 25(6), 061411 (Jul 21, 2016). ; http://dx.doi.org/10.1117/1.JEI.25.6.061411


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