High-quality three-dimensional reconstruction and noise reduction of multifocal images from oversized samples

Dalibor Martišek; Jana Procházková; Tomáš Ficker

doi:10.1117/1.JEI.24.5.053029

20 October 2015 High-quality three-dimensional reconstruction and noise reduction of multifocal images from oversized samples

Dalibor Martišek, Jana Procházková, Tomáš Ficker

Author Affiliations +

Journal of Electronic Imaging, Vol. 24, Issue 5, 053029 (October 2015). https://doi.org/10.1117/1.JEI.24.5.053029

Abstract

Three-dimensional (3-D) reconstruction is an indispensable tool in areas such as biology, chemistry, medicine, material sciences, etc. The sample can be reconstructed using confocal or nonconfocal mode of a microscope. The limitation of the confocal approach is the sample size. Currently used devices work mostly with sample surface area up to 1 cm². We suggest a three-step method that creates 3-D reconstruction from multifocal images in nonconfocal mode that is qualitatively comparable to the confocal results. Our method, thus, takes advantage of both microscope modes—high-quality results without sample size limitation. The preprocessing step eliminates the additive noise with Linderberg-Lévi theorem. The main focus criterion is based on adjusted Fourier transform. In the final step, we eliminate the defective clusters using the adaptive pixel neighborhood algorithm. We proved the effectiveness of our noise reduction and 3-D reconstruction method by the statistical comparisons; the correlation coefficients average 0.987 for all types of Fourier transforms.

Citation Download Citation

Dalibor Martišek, Jana Procházková, and Tomáš Ficker "High-quality three-dimensional reconstruction and noise reduction of multifocal images from oversized samples," Journal of Electronic Imaging 24(5), 053029 (20 October 2015). https://doi.org/10.1117/1.JEI.24.5.053029

Published: 20 October 2015

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available