The usual tomography is achieved by acquiring measurements around an object with multiple angles. The possibility of obtaining a fluorescence tomographic image from measurements at only one angle is explored. Instead of rotating around the object, the camera (or the objective lens) moves toward (or away from) the object and takes photographs while the camera’s focal plane passes through the object. The volume of stacked two-dimensional pictures forms a blurred three-dimensional image. The true image can be obtained by deconvolving the system’s point spread function. Simplified computer simulations are carried out to verify the feasibility of the proposed method. The computer simulations indicate that it is feasible to obtain a tomographic image by using the in-and-out motion to acquire data.